Comparison of Conventional Plating and Simplate Methods for Enumeration of Aerobic Microorganisms, Coliform and Escherichia Coli in Farm Environmental Samples
نویسندگان
چکیده
The objective of this study was to compare conventional plating to SimPlate methods for enumeration of aerobic microorganisms, coliforms and Escherichia coli in samples from farm animals and their environment. Samples evaluated included rectal swabs, fecal material, bedding, litter and feed and soil from beef and dairy cattle, swine and poultry farms. Standard method agar pour plating and conventional violet red bile agar with 4-methylumbelliferyl b-D-glucuronide were compared to the SimPlate total plate count-color indicator method for aerobic counts and the SimPlate coliform and E. coli color indicator method for total coliforms and E. coli counts, respectively. Overall, the SimPlate methods produced counts that were not significantly different (P 0.05) from those of the conventional methods. Population counts from the SimPlate methods were highly correlated with colony-forming unit counts from the conventional methods with r = 0.90, 0.94 and 0.94 for aerobic counts, total coliforms and E. coli, respectively. Thus, the data from this study indicate that SimPlate methods are comparable and reliable for enumeration of aerobic microorganisms, coliforms and E. coli in environmental farm samples.
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